Optimization of a direct real-time quantitative reverse transcription polymerase reaction (DRT-qPCR) assay for the detection of grapevine rupestris stem-pitting associated viruses (GRSPaV) in grapevine-Reference-Cited by-同舟云学术

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Optimization of a direct real-time quantitative reverse transcription polymerase reaction (DRT-qPCR) assay for the detection of grapevine rupestris stem-pitting associated viruses (GRSPaV) in grapevine

Published:2019-09-06 Issue:2 Volume:42 Page:292-303
ISSN:0706-0661
Container-title:Canadian Journal of Plant Pathology
language:en
Short-container-title:Canadian Journal of Plant Pathology

Author:

Greig N.¹,Luong J.¹,Hooker J.²,Stobbs L. W.¹,Meng B.²

Affiliation:

1. London Ontario Research and Development Centre, Agriculture and Agri-Food Canada, 4902 Victoria Ave. N., Vineland Station, ON L0R 2E0, Canada

2. Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, Canada

Funder

Agriculture and AgriFood Canada

Publisher

Informa UK Limited

Subject

Plant Science,Agronomy and Crop Science

Link

https://www.tandfonline.com/doi/pdf/10.1080/07060661.2019.1655483

Reference49 articles.

1. Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan® real-time RT-PCR

2. High prevalence of viruses in table grape from Spain detected by real-time RT-PCR

3. Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI

4. The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

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