Efficient screening of environmental isolates for Saccharomyces cerevisiae strains that are suitable for brewing-Reference-Cited by-同舟云学术

Efficient screening of environmental isolates for Saccharomyces cerevisiae strains that are suitable for brewing

Published:2014-06-03 Issue:6 Volume:78 Page:1086-1089
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Fujihara Hidehiko¹,Hino Mika¹,Takashita Hideharu²,Kajiwara Yasuhiro²,Okamoto Keiko²,Furukawa Kensuke¹

Affiliation:

1. Faculty of Food and Nutrition, Department of Food and Fermentation Science, Beppu University, Oita, Japan

2. Research & Development Laboratory, Sanwa Shurui Co. Ltd., Oita, Japan

Abstract

Abstract We developed an efficient screening method for Saccharomyces cerevisiae strains from environmental isolates. MultiPlex PCR was performed targeting four brewing S. cerevisiae genes (SSU1, AWA1, BIO6, and FLO1). At least three genes among the four were amplified from all S. cerevisiae strains. The use of this method allowed us to successfully obtain S. cerevisiae strains.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/78/6/1086/36798825/bbb1086.pdf

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