Multicopy integration and expression of heterologous genes in the oleaginous yeast, Lipomyces starkeyi-Reference-Cited by-同舟云学术

Multicopy integration and expression of heterologous genes in the oleaginous yeast, Lipomyces starkeyi

Published:2015-03-04 Issue:3 Volume:79 Page:512-515
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Oguro Yoshifumi¹,Yamazaki Harutake²,Shida Yosuke²,Ogasawara Wataru²,Takagi Masamichi¹,Takaku Hiroaki¹

Affiliation:

1. Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan

2. Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan

Abstract

Abstract The oleaginous yeast, Lipomyces starkeyi, is an excellent lipid producer with great industrial potential. However, methods for molecular breeding have not been established for L. starkeyi. We describe the development of a system for targeted rDNA integration of multiple copies of a gene into L. starkeyi genome by spheroplast–polyethylene glycol transformation.

Funder

Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/79/3/512/36806580/bbb0512.pdf

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