An optimized transformation protocol for Lipomyces starkeyi
Author:
Publisher
Springer Science and Business Media LLC
Subject
Genetics,General Medicine
Link
http://link.springer.com/content/pdf/10.1007/s00294-014-0427-0.pdf
Reference20 articles.
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2. Berardi E, Thomas DY (1990) An effective transformation method for Hansenula polymorpha. Curr Genet 18:169–170. doi: 10.1007/BF00312606
3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6:343–345. doi: 10.1038/nmeth.1318
4. Gietz RD, Schiestl RH (2007) High-efficiency yeast transformation using the LiAc/ss carrier DNA/PEG method. Nat Protoc 2:31–34. doi: 10.1038/nprot.2007.13
5. Gietz RD, Woods RA (2002) Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 350:87–96
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