Production of d-lactate using a pyruvate-producing Escherichia coli strain-Reference-Cited by-同舟云学术

Production of d-lactate using a pyruvate-producing Escherichia coli strain

Published:2017-07-03 Issue:7 Volume:81 Page:1452-1455
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Akita Hironaga¹,Nakashima Nobutaka²³,Hoshino Tamotsu¹²

Affiliation:

1. Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science Technology (AIST), Higashi-Hiroshima, Japan

2. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan

3. Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Ookayama, Japan

Abstract

Abstract To generate an organism capable of producing d-lactate, NAD+-dependent d-lactate dehydrogenase was expressed in our pyruvate-producing strain, Escherichia coli strain LAFCPCPt-accBC-aceE. After determining the optimal culture conditions for d-lactate production, 18.4 mM d-lactate was produced from biomass-based medium without supplemental mineral or nitrogen sources. Our results show that d-lactate can be produced in simple batch fermentation processes.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/81/7/1452/36823676/bbb1452.pdf

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