Enzymatic activity of cell-free extracts from Burkholderia oxyphila OX-01 bio-converts (+)-catechin and (−)-epicatechin to (+)-taxifolin-Reference-Cited by-同舟云学术

Enzymatic activity of cell-free extracts from Burkholderia oxyphila OX-01 bio-converts (+)-catechin and (−)-epicatechin to (+)-taxifolin

Published:2016-12-01 Issue:12 Volume:80 Page:2473-2479
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Otsuka Yuichiro¹²,Matsuda Motoki³,Sonoki Tomonori⁴,Sato-Izawa Kanna⁵,Goodell Barry²,Jelison Jody⁶,Navarro Ronald R¹,Murata Hitoshi⁷,Nakamura Masaya¹

Affiliation:

1. Department of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Japan

2. Department of Sustainable and Biomaterials, Virginia Polytechnic and State University, Blacksburg, VA, USA

3. Department of Biotechnology, Toyama Prefectural University, Imizu, Japan

4. Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan

5. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan

6. Center for Agriculture, Food and the Environment, University of Massachusetts Amherst, Amherst, MA, USA

7. Department of Mushroom Science and Forest Microbiology, Forestry and Forest Products Research Institute, Tsukuba, Japan

Abstract

Abstract This study characterized the enzymatic ability of a cell-free extract from an acidophilic (+)-catechin degrader Burkholderia oxyphila (OX-01). The crude OX-01 extracts were able to transform (+)-catechin and (−)-epicatechin into (+)-taxifolin via a leucocyanidin intermediate in a two-step oxidation. Enzymatic oxidation at the C-4 position was carried out anaerobically using H2O as an oxygen donor. The C-4 oxidation occurred only in the presence of the 2R-catechin stereoisomer, with the C-3 stereoisomer not affecting the reaction. These results suggest that the OX-01 may have evolved to target both (+)-catechin and (−)-epicatechin, which are major structural units in plants.

Funder

The Grant-in-Aid for Young Scientists (B) of KAKENHI

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/80/12/2473/36829470/bbb2473.pdf

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