Expression of a codon-optimized Aspergillus niger pectin methylesterase gene in the methylotrophic yeast Candida boidinii-Reference-Cited by-同舟云学术

Expression of a codon-optimized Aspergillus niger pectin methylesterase gene in the methylotrophic yeast Candida boidinii

Published:2014-04-03 Issue:4 Volume:78 Page:718-721
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Kawaguchi Kosuke¹²,Yurimoto Hiroya¹,Sakai Yasuyoshi¹³

Affiliation:

1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto, Japan

2. Graduate School of Medicine and Pharmaceutical Sciences, Department of Biological Chemistry, University of Toyama, Toyama, Japan

3. Advanced Low Carbon Technology Research and Development Program, Japan Science and Technology Agency, Chiyoda-ku, Tokyo, Japan

Abstract

Abstract A codon-optimized Aspergillus niger pectin methylesterase (PME) gene was expressed in the methylotrophic yeast Canidia boidinii. The PME-producing strains showed better growth on pectin than the wild-type strains, suggesting that the PME-producing strains could efficiently utilize methyl ester moieties of pectin. On the other hand, overproduction of PME negatively affected the proliferation of C. boidinii on leaves of Arabidopsis thaliana.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/78/4/718/36797327/bbb0718.pdf

Reference22 articles.

1. Detection of substantial emissions of methanol from plants to the atmosphere;MacDonald;Atmos. Environ. Part A: Gen. Top,1993

2. Methanol emission from leaves (Enzymatic detection of gas-phase methanol and relation of methanol fluxes to stomatal conductance and leaf development);Nemecek-Marshall;Plant Physiol,1995

3. Leaf methanol - the simplest natural product from plants;Fall;Trends Plant Sci,1996

4. The production of methanol by flowering plants and the global cycle of methanol;Galbally;J. Atmos. Chem,2002

5. Methanol accumulation in maturing seeds;Obendorf;J. Exp. Bot,1990

Cited by 5 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The effect of Aspergillus luchuensis pectin methylesterase genes pmeA and pmeB on methanol production in sweet potato shochu;Bioscience, Biotechnology, and Biochemistry;2023-04-25

2. Yeast Cell Sensor: Single-Cell Technology for Methanol DetectionIts Use in Biological Researches, Environmental Sciences, and Enzyme Engineering;KAGAKU TO SEIBUTSU;2020-07-01

3. Expression and characterization of a pectin methylesterase from Aspergillus niger ZJ5 and its application in fruit processing;Journal of Bioscience and Bioengineering;2018-12

4. A Pichia pastoris single-cell biosensor for detection of enzymatically produced methanol;Applied Microbiology and Biotechnology;2018-06-11

5. Methylotrophic Yeasts as Producers of Recombinant Proteins;Biotechnology of Yeasts and Filamentous Fungi;2017