Affiliation:
1. Bio-Process Engineering Laboratory, School of Engineering & Graduate School of Medicine, Yamaguchi University Biomedical Engineering Center (YUBEC), Yamaguchi University, Ube, Japan
Abstract
Abstract
A simple method for calculating the productivity of chromatography processes was proposed based on the iso-resolution curve concept. The model separation system was polyphenol separations by polystyrene divinylbenzene resins with the ethanol–water mixture mobile phase. The distribution coefficient K was determined as a function of ethanol concentration I by linear gradient elution experiments. The HETP-mobile phase velocity u curves were determined as a function of I. Using K and HETP, the iso-resolution curve was calculated, from which the productivity was determined as a function of I. It was found that there is an optimum I, where the highest productivity with the minimum amount of mobile phase consumption is obtained.
Funder
Ministry of Economy, Trade and Industry, Japan
Japan Agency for Medical Research and Development
Publisher
Oxford University Press (OUP)
Subject
Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology
Cited by
13 articles.
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