Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR-Reference-Cited by-同舟云学术

Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR

Published:2017-01-25 Issue:2 Volume:31 Page:339-348
ISSN:1310-2818
Container-title:Biotechnology & Biotechnological Equipment
language:en
Short-container-title:Biotechnology & Biotechnological Equipment

Author:

Zeng Fanli¹²,Zhang Yujie¹,Zhang Ze¹,Malik Asrar Ahmad²,Lin Yibin²³

Affiliation:

1. College of Life Sciences, Agricultural University of Hebei, Baoding, P.R. China

2. Biophysics Unit, Department of Biochemistry and Molecular Biology, Medical School, and Center of Biophysics, Autonomous University of Barcelona, Barcelona, Spain

3. Department of Biochemistry and Molecular Biology, Center for Membrane Biology, The University of Texas Houston Medical School, Houston, TX, USA

Funder

Agricultural University of Hebei

Publisher

Informa UK Limited

Subject

Biotechnology

Link

https://tandfonline.com/doi/pdf/10.1080/13102818.2017.1279033

Reference29 articles.

1. A general method ofin vitropreparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

2. Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest

3. Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h

4. Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Gene Mutagenesis Methods in Directed Evolution and Rational Enzyme Design;Enzyme Engineering;2023-01-06

2. Pantoea ananatis carotenoid production confers toxoflavin tolerance and is regulated by Hfq‐controlled quorum sensing;MicrobiologyOpen;2020-12-02

3. ITS2 Pretrial Gene Identification Related to Seed and Flower Identification for Cyclea barbata;2020-08-21

4. Rice Tolerance to Drought is Complex Both Physiologically and Genetically;2020-08-20

5. The heterotrimeric G protein г Stgg1 is required for conidiation, secondary metabolite production and pathogenicity of Setosphaeria turcica;Biotechnology & Biotechnological Equipment;2018-06-28