Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest-Reference-Cited by-同舟云学术

Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest

Published:1997-02 Issue:1 Volume:186 Page:29-35
ISSN:0378-1119
Container-title:Gene
language:en
Short-container-title:Gene

Author:

Warrens Anthony N,Jones Michael D,Lechler Robert I

Publisher

Elsevier BV

Subject

Genetics,General Medicine

Reference13 articles.

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2. Bell, J., Estess, P., St John, T., Saiki, R., Watling, D., Erich, H. and McDevitt, H. (1985) DNA sequence and characterisation of human class II major histocompatibility complex β chain from the DR1 haplotype. Proc. Natl. Acad. Sci. USA 82, 3405–3409.

3. Brown, J.H., Jardetzky, T.S., Gorga, J.C., Stern, L.J., Urban, R.G., Strominger, J.L. and Wiley, D.C. (1993) Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1. Nature 364, 33–39.

4. Clark, J., Joyce, C. and Beardsley, G. (1987) Novel blunt-end addition reactions catalysed by DNA polymerases. J. Mol. Biol. 198, 123–127.

5. Horton, R.M., Hunt, H.D., Ho, S.N., Pullen, J.K. and Pease, L.R. (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77, 61–68.

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