High GC Contents of Primer 5′-End Increases Reaction Efficiency in Polymerase Chain Reaction-Reference-Cited by-同舟云学术

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High GC Contents of Primer 5′-End Increases Reaction Efficiency in Polymerase Chain Reaction

Published:2009-05-22 Issue:4 Volume:28 Page:324-330
ISSN:1525-7770
Container-title:Nucleosides, Nucleotides and Nucleic Acids
language:en
Short-container-title:Nucleosides, Nucleotides and Nucleic Acids

Author:

Shinoda Naoki,Yoshida Tomotaro,Kusama Toyoko,Takagi Masami,Hayakawa Toshiaki,Onodera Takashi,Sugiura Katsuaki

Publisher

Informa UK Limited

Subject

Genetics,Molecular Medicine,Biochemistry,General Medicine

Link

http://www.tandfonline.com/doi/pdf/10.1080/15257770902963400

Reference19 articles.

1. [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

2. Primer design for large scale sequencing

3. Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

4. Amplification of GC-rich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions

5. Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases

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