High-resolution mass spectrometry confirms the presence of a hydroxyproline (Hyp) post-translational modification in the GGGGP linker of an Fc-fusion protein-Reference-Cited by-同舟云学术

High-resolution mass spectrometry confirms the presence of a hydroxyproline (Hyp) post-translational modification in the GGGGP linker of an Fc-fusion protein

Published:2017-05-16 Issue:5 Volume:9 Page:812-819
ISSN:1942-0862
Container-title:mAbs
language:en
Short-container-title:mAbs

Author:

Spahr Chris¹^ORCID,Gunasekaran Kannan²,Walker Kenneth W.²,Shi Stone D.-H.¹^ORCID

Affiliation:

1. Discovery Attribute Sciences, Therapeutic Discovery, Amgen Inc., Thousand Oaks, CA, USA

2. Biologics Optimization, Therapeutic Discovery, Amgen Inc., Thousand Oaks, CA, USA

Publisher

Informa UK Limited

Subject

Immunology,Immunology and Allergy

Link

https://www.tandfonline.com/doi/pdf/10.1080/19420862.2017.1325556

Reference29 articles.

1. Linkers in the structural biology of protein-protein interactions

2. Fusion protein linkers: Property, design and functionality

3. Alternative molecular formats and therapeutic applications for bispecific antibodies

4. Molecular construction and optimization of anti-human IL-1α/β dual variable domain immunoglobulin (DVD-IgTM) molecules

5. Ligand association rates to the inner-variable-domain of a dual-variable-domain immunoglobulin are significantly impacted by linker design

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