Activating the branch-forming splicing pathway by reengineering the ribozyme component of a natural group II intron

Author:

Monachello Dario,Michel François,Costa Maria

Abstract

When assayed in vitro, group IIC self-splicing introns, which target bacterial Rho-independent transcription terminators, generally fail to yield branched products during splicing despite their possessing a seemingly normal branchpoint. Starting with intron O.i.I1 from Oceanobacillus iheyensis, whose crystallographically determined structure lacks branchpoint-containing domain VI, we attempted to determine what makes this intron unfit for in vitro branch formation. A major factor was found to be the length of the helix at the base of domain VI: 4 base pairs (bp) are required for efficient branching, even though a majority of group IIC introns have a 3-bp helix. Equally important for lariat formation is the removal of interactions between ribozyme domains II and VI, which are specific to the second step of splicing. Conversely, mismatching of domain VI and its proposed first-step receptor in subdomain IC1 was found to be detrimental; these data suggest that the intron-encoded protein may promote branch formation partly by modulating the equilibrium between conformations specific to the first and second steps of splicing. As a practical application, we show that by making just two changes to the O.i.I1 ribozyme, it is possible to generate sufficient amounts of lariat intron for the latter to be purified and used in kinetic assays in which folding and reaction are uncoupled.

Funder

French Agence Nationale de la Recherche

Publisher

Cold Spring Harbor Laboratory

Subject

Molecular Biology

Cited by 10 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Protein-Free Catalysis of DNA Hydrolysis and Self-Integration by a Ribozyme;2024-08-31

2. Ribozymes as Therapeutic Agents Against Infectious Diseases;RNA Therapeutics - History, Design, Manufacturing, and Applications [Working Title];2022-09-29

3. Mechanisms of catalytic RNA molecules;Biochemical Society Transactions;2021-08-20

4. Transitions between the steps of forward and reverse splicing of group IIC introns;RNA;2020-03-03

5. The mechanism of splicing as told by group II introns: Ancestors of the spliceosome;Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms;2019-11

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