Exploring the variability of root system architecture under drought stress in heat-tolerant spring-wheat lines

Abstract

Abstract Background and aims Global wheat production is under threat due to climate change, specifically heat and drought and their combination. This study aims to address the root trait responses of heat-tolerant wheat genotypes to drought. Methods The variability in root traits of CIMMYT wheat lines, which were previously developed for heat stress tolerance (HTWL), was evaluated alongside 10 Pakistani-approved varieties under three cultivation conditions and soil moisture levels. Results Our findings revealed that the plasticity of the wheat root system is highly pronounced, with rhizosphere conditions exerting a more substantial influence (5–49%) than the genotypic response (1–14%). Furthermore, in the hydroponic and pot system, we noted higher maximum-root length (1.5–1.8 fold) and root-to-shoot ratio (3.4–10.6 fold) as compared to field condition, while the root biomass was substantially higher in the field trial (3-57 fold). Nonetheless, persistent drought conditions exerted contrasting impact with reduction in most of the traits except specific root length and harvest index which were increased under drought. Conclusions The variation in root traits against drought indicates the potential for the development of improved genotypes that can withstand multiple stresses. Furthermore, it is crucial to consider rhizosphere conditions when selecting genotypes, as the plasticity of wheat roots may lead to misinterpretations if rhizosphere conditions are disregarded. Root dry weight and root-to-shoot ratio are more stable traits as compared to maximum root length and specific root length. It is recommended to evaluate a broader range of rhizosphere conditions to select tolerant genotypes.