Affordable uniform isotope labeling with 2H, 13C and 15N in insect cells
Author:
Publisher
Springer Science and Business Media LLC
Subject
Spectroscopy,Biochemistry
Link
http://link.springer.com/content/pdf/10.1007/s10858-015-9935-6.pdf
Reference21 articles.
1. Brüggert M, Rehm T, Shanker S et al (2003) A novel medium for expression of proteins selectively labeled with 15 N-amino acids in Spodoptera frugiperda (Sf9) insect cells. J Biomol NMR 25:335–348. doi: 10.1023/A:1023062906448
2. Dong S, Wang M, Qiu Z et al (2010) Autographa californica multicapsid nucleopolyhedrovirus efficiently infects Sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low pH. J Virol 84:5351–5359. doi: 10.1128/JVI.02517-09
3. Doverskog M, Han L, Häggström L (1998) Cystine/cysteine metabolism in cultured Sf9 cells: influence of cell physiology on biosynthesis, amino acid uptake and growth. Cytotechnology 26:91–102. doi: 10.1023/A:1007963003607
4. Egorova-Zachernyuk TA, Bosman GJCGM, Pistorius AMA, DeGrip WJ (2009) Production of yeastolates for uniform stable isotope labelling in eukaryotic cell culture. Appl Microbiol Biotechnol 84:575–581. doi: 10.1007/s00253-009-2063-z
5. Gossert AD, Jahnke W (2012) Isotope labeling in insect cells. In: Atreya HS (ed) Isotope labeling in biomolecular NMR. Springer, Dordrecht, pp 179–196
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