Bacterial expression and one-step purification of an isotope-labeled heterotrimeric G-protein α-subunit-Reference-Cited by-同舟云学术

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Bacterial expression and one-step purification of an isotope-labeled heterotrimeric G-protein α-subunit

Published:2005-05 Issue:1 Volume:32 Page:31-40
ISSN:0925-2738
Container-title:Journal of Biomolecular NMR
language:en
Short-container-title:J Biomol NMR

Author:

Abdulaev Najmoutin G.,Zhang Cheng,Dinh Andy,Ngo Tony,Bryan Philip N.,Brabazon Danielle M.,Marino John P.,Ridge Kevin D.

Publisher

Springer Science and Business Media LLC

Subject

Spectroscopy,Biochemistry

Link

http://link.springer.com/content/pdf/10.1007/s10858-005-3340-5.pdf

Reference41 articles.

1. Functionally Discrete Mimics of Light-activated Rhodopsin Identified through Expression of Soluble Cytoplasmic Domains

2. Characterization of the aluminum and beryllium fluoride species which activate transducin. Analysis of the binding and dissociation kinetics.

3. Recombinant protein expression in Escherichia coli

4. Solution Structure of the GTPase Activating Domain of αs

5. Complete 1H, 13C, and 15N Assignments and Secondary Structure of the GTPase Activating Domain of Gs

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1. In-depth analysis of Gαs protein activity by probing different fluorescently labeled guanine nucleotides;Biological Chemistry;2024-02-02

2. Evidence that the Rhodopsin Kinase (GRK1) N-Terminus and the Transducin Gα C-Terminus Interact with the Same “Hydrophobic Patch” on Rhodopsin TM5;Biochemistry;2016-05-26

3. Distance Mapping in Proteins Using Fluorescence Spectroscopy: Tyrosine, like Tryptophan, Quenches Bimane Fluorescence in a Distance-Dependent Manner;Biochemistry;2014-10-01

4. Overview of Affinity Tags for Protein Purification;Current Protocols in Protein Science;2013-08

5. Backbone resonance assignments for G protein αi3 subunit in the GDP-bound state;Biomolecular NMR Assignments;2013-06-15

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