A simple protocol for expression of isotope-labeled proteins in Escherichia coli grown in shaker flasks at high cell density
Author:
Funder
NIDDK
Publisher
Springer Science and Business Media LLC
Subject
Spectroscopy,Biochemistry
Link
http://link.springer.com/content/pdf/10.1007/s10858-019-00285-x.pdf
Reference9 articles.
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3. Cai M, Huang Y, Sakaguchi K, Clore GM, Gronenborn AM, Craigie R (1998) An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli. J Biomol NMR 11:97–102
4. Cai M, Huang Y, Yang R, Craigie R, Clore GM (2016) A simple and robust protocol for high-yield expression of perdeuterated proteins in Escherichia coli grown in shaker flasks. J Biomol NMR 66:85–91
5. Collins T, Azevedo-Silva J, da Costa A, Branca F, Machado R, Casal M (2013) Batch production of a silk-elastin-like protein in E. coli BL21 (DE3): key parameters for optimization. Microb Cell Fact 12:21
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