A simple and robust protocol for high-yield expression of perdeuterated proteins in Escherichia coli grown in shaker flasks
Author:
Funder
NIDDK, National Institutes of Health
Publisher
Springer Science and Business Media LLC
Subject
Spectroscopy,Biochemistry
Link
http://link.springer.com/content/pdf/10.1007/s10858-016-0052-y.pdf
Reference7 articles.
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4. Duff AP, Wilde KL, Rekas A, Lake V, Holden PJ (2015) Robust high-yield methodologies for 2H and 2H/15N/13C labeling of proteins for structural investigations using neutron scattering and NMR. Meth Enzymol 565:3–25
5. Gardner KH, Kay LE (1998) The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins. Annu Rev Biophys Biomol Struct 27:357–406
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