Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency-Reference-Cited by-同舟云学术

Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency

Published:2014-01-14 Issue:13-14 Volume:181 Page:1689-1696
ISSN:0026-3672
Container-title:Microchimica Acta
language:en
Short-container-title:Microchim Acta

Author:

Ruijter Jan M.,Lorenz Peter,Tuomi Jari M.,Hecker Michael,van den Hoff Maurice J. B.

Publisher

Springer Science and Business Media LLC

Subject

Analytical Chemistry

Link

http://link.springer.com/content/pdf/10.1007/s00604-013-1155-8.pdf

Reference20 articles.

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3. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR (2005) Basic principles of real-time quantitative PCR. Expert Rev Mol Diagn 5:209–219

4. Ruijter JM, Pfaffl MW, Zhao S, Spiess AN, Boggy G, Blom J, Rutledge RG, Sisti D, Lievens A, De PK, Derveaux S, Hellemans J, Vandesompele J (2012) Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications. Methods 59:32–46

5. Ruijter JM, Ramakers C, Hoogaars WM, Karlen Y, Bakker O, van den Hoff MJ, Moorman AF (2009) Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Res 37:e45

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