In-House Validation and Comparison of Two Wheat (Triticum aestivum) Taxon-Specific Real-Time PCR Methods for GMO Quantification Supported by Droplet Digital PCR

Author:

Paternò Annalisa,Verginelli Daniela,Bonini Pamela,Misto Marisa,Quarchioni Cinzia,Dainese Enrico,Peddis Stefania,Fusco Cristiana,Vinciguerra Daniela,Marchesi UgoORCID

Funder

Ministero della Salute

Publisher

Springer Science and Business Media LLC

Subject

Safety Research,Safety, Risk, Reliability and Quality,Applied Microbiology and Biotechnology,Food Science,Analytical Chemistry

Reference37 articles.

1. Abouseadaa HH, Osman GH, Ramadan AM, Hassanein SE, Abdelsattar MT, Morsy YB, Alameldin HF, El-Ghareeb DK, Nour-Eldin HA, Salem R, Gad AA, Elkhodary SE, Shehata MM, Mahfouz HM, Eissa HF, Bahieldin A. (2015) Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects. BMC Plant Biol. 22;15:183. doi: https://doi.org/10.1186/s12870-015-0570-x

2. Anand A, Zhou T, Trick HN, Gill BS, Bockus WW, Muthukrishnan S (2003) Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum. J Exp Bot 54(384):1101–1111. https://doi.org/10.1093/jxb/erg110

3. Bennett MD, Smith JB. (1976) Nuclear DNA amounts in angiosperms. Philosophical Transactions of the Royal Society of London Series B-Biological Sciences 274: 227–274. Kew Royal Botanic Garden. http://data.kew.org/cvalues/

4. Burns MJ, Burrell AM, Foy CA (2010) The applicability of digital PCR for the assessment of detection limits in GMO analysis. Eur Food Res Technol 231(3):353–362. https://doi.org/10.1007/s00217-010-1277-8

5. Chaouachi M, El Malki R, Berard A, Romaniuk M, Laval V, Brunel D, Bertheau Y (2008) Development of a real-time PCR method for the differential detection and quantification of four Solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annum). J Agric Food Chem 56(6):1818–1828. https://doi.org/10.1021/jf073313n

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3