Von Kossa and his staining technique

Author:

Schneider Marlon R.ORCID

Abstract

AbstractOne hundred and twenty years ago, the Hungarian physician Julius von Kossa developed a now classical staining method for detecting mineral deposits in animal tissues. Since then, this method has been widely adapted and combined with different counterstains, but still bears the name of its original inventor, who, if alive, would have turned 150 in 2015. As a rather inexpensive technique that does not require special instrumentation, von Kossa’s staining method became extremely popular for demonstrating mineralized tissues in vivo and in vitro. This article pays tribute to von Kossa and to his handy staining method.

Funder

Universität Leipzig

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Medical Laboratory Technology,Molecular Biology,Histology

Reference11 articles.

1. Bonewald LF, Harris SE, Rosser J, Dallas MR, Dallas SL, Camacho NP, Boyan B, Boskey A (2003) von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone formation. Calcif Tissue Int 72(5):537–547

2. Estell EG, Le PT, Vegting Y, Kim H, Wrann C, Bouxsein ML, Nagano K, Baron R, Spiegelman BM, Rosen CJ (2020) Irisin directly stimulates osteoclastogenesis and bone resorption in vitro and in vivo. eLife 9:e58172

3. Karasszon D (1990) The 125th anniversary of the birth of Gyula Magyary-Kossa (1865–1944), the great master of Hungarian medical historiography. Orv Hetil 131(26):1427–1428

4. Klein U (2003) Experiments, models, paper tools—cultures of organic chemistry in the nineteenth century. Stanford University Press, Stanford

5. von Kossa J (1901) Ueber die im Organismus künstlich erzeugbaren Verkalkungen. Beiträge Zur Pathologischen Anatomie Und Zur Allgemeinen Pathologie 29:163–202

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