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Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR

  • Published:2018-12-08 Issue:3 Volume:64 Page:421-428
  • ISSN:0015-5632
  • Container-title:Folia Microbiologica
  • language:en
  • Short-container-title:Folia Microbiol

Author:

Ricna Dita,Lengerova Martina,Bezdicek Matej,Kocmanova Iva,Drgona Lubos,Weinbergerova Barbora,Mayer Jiri,Racil Zdenek

Funder

Ministerstvo Zdravotnictv? Cesk? Republiky

Technologick? Agentura ?esk? Republiky

Masarykova Univerzita

Publisher

Springer Science and Business Media LLC

Subject

General Medicine,Microbiology

Reference35 articles.

1. Alanio A, Bretagne S (2014) Difficulties with molecular diagnostic tests for mould and yeast infections: where do we stand? Clin Microbiol Infect 20:36–41. https://doi.org/10.1111/1469-0691.12617

2. Babouee B, Goldenberger D, Elzi L, Lardinois D, Sadowski-Cron C, Bubendorf L, Savic Prince S, Battegay M, Frei R, Weisser M (2013) Prospective study of a panfungal PCR assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: a complementary tool in the diagnosis of invasive fungal disease? Clin Microbiol Infect 19:354–357. https://doi.org/10.1111/1469-0691.12231

3. Bezdicek M, Lengerova M, Ricna D, Weinbergerova B, Kocmanova I, Volfova P, Drgona L, Poczova M, Mayer J, Racil Z (2016) Rapid detection of fungal pathogens in bronchoalveolar lavage samples using panfungal PCR combined with high resolution melting analysis. Med Mycol 54:714–724. https://doi.org/10.1093/mmy/myw032

4. Bittinger K, Charlson ES, Loy E, Shirley DJ, Haas AR, Laughlin A, Yi Y, Wu GD, Lewis JD, Frank I, Cantu E, Diamond JM, Christie JD, Collman RG, Bushman FD (2014) Improved characterization of medically relevant fungi in the human respiratory tract using next-generation sequencing. Genome Biol 15:487. https://doi.org/10.1186/s13059-014-0487-y

5. Boch T, Spiess B, Cornely OA, Vehreschild JJ, Rath PM, Steinmann J, Heinz WJ, Hahn J, Krause SW, Kiehl MG, Egerer G, Liebregts T, Koldehoff M, Klein M, Nolte F, Mueller MC, Merker N, Will S, Mossner M, Popp H, Hofmann WK, Reinwald M, Buchheidt D (2016) Diagnosis of invasive fungal infections in haematological patients by combined use of galactomannan, 1,3-β-D-glucan, Aspergillus PCR, multifungal DNA-microarray, and Aspergillus azole resistance PCRs in blood and bronchoalveolar lavage samples: results of a prospective multicentre study. Clin Microbiol Infect 22:862–868. https://doi.org/10.1016/j.cmi.2016.06.021
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