Enhancing the expression of recombinant small laccase in Pichia pastoris by a double promoter system and application in antibiotics degradation
Author:
Funder
National Research Foundation
Council of Scientific and Industrial Research
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Microbiology
Link
https://link.springer.com/content/pdf/10.1007/s12223-021-00894-w.pdf
Reference57 articles.
1. Antošová Z, Sychrová H (2016) Yeast hosts for the production of recombinant laccases: a review. Mol Biotechnol 58:93–116. https://doi.org/10.1007/s12033-015-9910-1
2. Becker D, Varela Della Giustina S, Rodriguez-Mozaz S et al (2016) Removal of antibiotics in wastewater by enzymatic treatment with fungal laccase – degradation of compounds does not always eliminate toxicity. Bioresour Technol 219:500–509. https://doi.org/10.1016/j.biortech.2016.08.004
3. Cámara E, Landes N, Albiol J et al (2017) Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris. Sci Rep 7:1–16. https://doi.org/10.1038/srep44302
4. Canales C, Altamirano C, Berrios J (2018) The growth of Pichia pastoris Mut+ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production. Bioprocess Biosyst Eng 41:1827–1838. https://doi.org/10.1007/s00449-018-2005-1
5. Cereghino GPL, Cereghino JL, Ilgen C, Cregg JM (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13:329–332. https://doi.org/10.1016/S0958166902003300
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