Author:
Cui Songkui,Ghanim Abdelbagi M. A.,Yoshida Satoko
Abstract
AbstractMolecular markers are powerful tools to enhance speed of breeding. Recent advances of sequencing technology allow us to identify most polymorphisms in a genome. Whole genome resequencing of an F2 segregating population can identify those polymorphisms that co-segregate with a mutant phenotype, which theoretically include the gene responsible for Striga resistance gained through mutagenesis. This protocol explains the isolation of high-quality genomic DNA for sequencing and step-by-step procedures for single nucleotide polymorphism (SNP) identification from bulked F2 sequences.
Publisher
Springer Berlin Heidelberg