High-level production of single chain monellin mutants with enhanced sweetness and stability in tobacco chloroplasts
Author:
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Genetics
Link
http://link.springer.com/article/10.1007/s00425-018-2920-z/fulltext.html
Reference71 articles.
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2. Boyhan D, Daniell H (2011) Low-cost production of proinsulin in tobacco and lettuce chloroplasts for injectable or oral delivery of functional insulin and C-peptide. Plant Biotechnol J 9:585–598
3. Cai C, Li L, Lu N, Zheng W, Yang L, Liu B (2016) Expression of a high sweetness and heat-resistant mutant of sweet-tasting protein, monellin, in Pichia pastoris with a constitutive GAPDH promoter and modified N-terminus. Biotechnol Lett 38:1941–1946
4. Castiglia D, Sannino L, Marcolongo L, Ionata E, Tamburino R, De Stradis A, Cobucci-Ponzano B, Moracci M, La Cara F, Scotti N (2016) High-level expression of thermostable cellulolytic enzymes in tobacco transplastomic plants and their use in hydrolysis of an industrially pretreated Arundo donax L. biomass. Biotechnol Biofuels 9:154
5. Chen Z, Heng C, Li Z, Liang X, Xinchen S (2007) Expression and secretion of a single-chain sweet protein monellin in Bacillus subtilis by sacB promoter and signal peptide. Appl Microbiol Biotechnol 73:1377–1381
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