Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8+ T cells with detection by ELISPOT and HLA-multimer staining

Author:

Chudley Lindsey,McCann Katy J.,Coleman Adam,Cazaly Angelica M.,Bidmon Nicole,Britten Cedrik M.,van der Burg Sjoerd H.,Gouttefangeas Cecile,Jandus Camilla,Laske Karoline,Maurer Dominik,Romero Pedro,Schröder Helene,Stynenbosch Linda F. M.,Walter Steffen,Welters Marij J. P.,Ottensmeier Christian H.

Publisher

Springer Science and Business Media LLC

Subject

Cancer Research,Oncology,Immunology,Immunology and Allergy

Reference24 articles.

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2. Schloot NC, Meierhoff G, Karlsson Faresjö M, Ott P, Putnam A, Lehmann P, Gottlieb P, Roep BO, Peakman M, Tree T (2003) Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop. J Autoimmun 21:365–376

3. Scheibenbogen C, Romero P, Rivoltini L, Herr W, Schmittel A, Cerottini JC, Woelfel T, Eggermont AM, Keilholz U (2000) Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial. J Immunol Methods 244:81–89

4. Smith SG, Joosten SA, Verscheure V, Pathan AA, McShane H, Ottenhoff TH, Dockrell HM, Mascart F (2009) Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from Mycobacterium tuberculosis. PLoS ONE 4:e7972

5. Cox, J. H., Ferrari, G., Kalams, S. A., Lopaczynski, W., Oden, N., D’souza, M. P., Group, E. C. S (2005) Results of an ELISPOT proficiency panel conducted in 11 laboratories participating in international human immunodeficiency virus type 1 vaccine trials. AIDS Res Hum Retroviruses 21:68–81

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