An active-site carboxyl group in liquefying α-amylase: specific chemical modification-Reference-Cited by-同舟云学术

An active-site carboxyl group in liquefying α-amylase: specific chemical modification

Published:1984-07-01 Issue:7 Volume:4 Page:613-619
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Kochhar Sunil¹,Dua R. D.¹

Affiliation:

1. Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Hauz Khas, New Delhi – 110 016, India

Abstract

Bacillus amyloliquefaciens α-amylase activity is pH-dependent and the plot log (Vmax/Km) versus pH implicated a carboxyl group of aspartic acid/glutamic acid at the active site. Chemical modification of α-amylase with EDC confirmed this view. Further, analysis of inactivation kinetics showed that modification of a single carboxyl group led to complete loss of the enzymic activity.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/4/7/613/471227/bsr0040613.pdf

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