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An efficient Agrobacterium-mediated transformation method using hypocotyl as explants for Brassica napus

  • Published:2020-09-28 Issue:10 Volume:40 Page:
  • ISSN:1380-3743
  • Container-title:Molecular Breeding
  • language:en
  • Short-container-title:Mol Breeding

Author:

Dai Cheng,Li Yuqing,Li Long,Du Zhuolin,Lin Shengli,Tian Xia,Li Sijia,Yang Bao,Yao Wei,Wang Jing,Guo Liang,Lu Shaoping

Funder

National Program of Transgenic Variety Development of China

National Natural Science Foundation of China

Fundamental Research Funds for the Central Universities

China Postdoctoral Science Foundation

Publisher

Springer Science and Business Media LLC

Subject

Plant Science,Genetics,Agronomy and Crop Science,Molecular Biology,Biotechnology

Reference39 articles.

1. Berg DE, Davies J, Allet B, Rochaix JD (1975) Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci U S A 72(9):3628–3632. https://doi.org/10.1073/pnas.72.9.3628

2. Bergman P, Glimelius K (1993) Electroporation of rapeseed protoplasts – transient and stable transformation. 88(4):604–611. https://doi.org/10.1111/j.1399-3054.1993.tb01378.x

3. Bhalla PL, Singh MB (2008) Agrobacterium-mediated transformation of Brassica napus and Brassica oleracea. Nat Protoc 3(2):181–189. https://doi.org/10.1038/nprot.2007.527

4. Chalhoub B, Denoeud F, Liu S, Parkin IA, Tang H, Wang X, Chiquet J, Belcram H, Tong C, Samans B, Correa M, Da Silva C, Just J, Falentin C, Koh CS, Le Clainche I, Bernard M, Bento P, Noel B, Labadie K, Alberti A, Charles M, Arnaud D, Guo H, Daviaud C, Alamery S, Jabbari K, Zhao M, Edger PP, Chelaifa H, Tack D, Lassalle G, Mestiri I, Schnel N, Le Paslier MC, Fan G, Renault V, Bayer PE, Golicz AA, Manoli S, Lee TH, Thi VH, Chalabi S, Hu Q, Fan C, Tollenaere R, Lu Y, Battail C, Shen J, Sidebottom CH, Wang X, Canaguier A, Chauveau A, Berard A, Deniot G, Guan M, Liu Z, Sun F, Lim YP, Lyons E, Town CD, Bancroft I, Wang X, Meng J, Ma J, Pires JC, King GJ, Brunel D, Delourme R, Renard M, Aury JM, Adams KL, Batley J, Snowdon RJ, Tost J, Edwards D, Zhou Y, Hua W, Sharpe AG, Paterson AH, Guan C, Wincker P (2014) Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science 345(6199):950–953. https://doi.org/10.1126/science.1253435

5. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16(6):735–743. https://doi.org/10.1046/j.1365-313x.1998.00343.x
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