Insertion orientation within the cassette affects gene-targeting success during ends-out recombination in the yeast Saccharomyces cerevisiae
Author:
Funder
Ministarstvo Obrazovanja, Znanosti i Sporta
Publisher
Springer Science and Business Media LLC
Subject
Genetics,General Medicine
Link
https://link.springer.com/content/pdf/10.1007/s00294-022-01246-y.pdf
Reference48 articles.
1. Aguilera A, Gaillard H (2014) Transcription and recombination: when RNA meets DNA. Cold Spring Harb Perspect Biol 6:a016543. https://doi.org/10.1101/cshperspect.a016543
2. Amberg DC, Botstein D, Beasley EM (1995) Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction. Yeast 11:1275–1280. https://doi.org/10.1002/yea.320111307
3. Astromskas E, Cohn M (2009) Ends-in vs. ends-out targeted insertion mutagenesis in Saccharomyces castellii. Curr Genet 55:339–347. https://doi.org/10.1007/s00294-009-0248-8
4. Baganz F, Hayes A, Marren D, Gardner DC, Oliver SG (1997) Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae. Yeast 13:1563–1573. https://doi.org/10.1002/(SICI)1097-0061(199712)13:16%3c1563::AID-YEA240%3e3.0.CO;2-6
5. Bailis AM, Maines S (1996) Nucleotide excision repair gene function in short-sequence recombination. J Bacteriol 178:2136–2140. https://doi.org/10.1128/jb.178.7.2136-2140.1996
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1. Unilateral ends‐out gene targeting increases mistargeting through supporting extensive single‐strand assimilation;Yeast;2023-10-08
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