RT-qPCR with chimeric dU stem-loop primer is efficient for the detection of bacterial small RNAs-Reference-Cited by-同舟云学术

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RT-qPCR with chimeric dU stem-loop primer is efficient for the detection of bacterial small RNAs

Published:2017-03-17 Issue:11 Volume:101 Page:4561-4568
ISSN:0175-7598
Container-title:Applied Microbiology and Biotechnology
language:en
Short-container-title:Appl Microbiol Biotechnol

Author:

Wu Yangfan,Xing Xuejiao,You Ting,Liang Rubing,Liu Jianhua

Funder

National Natural Science Foundation of China

Shanghai Pujiang Program

Chen Xing Grant of Shanghai Jiaotong University

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

http://link.springer.com/article/10.1007/s00253-017-8181-0/fulltext.html

Reference30 articles.

1. Altuvia S (2007) Identification of bacterial small non-coding RNAs: experimental approaches. Curr Opin in Microbiol 10:257–261

2. Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ (2005) Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 33:e179

3. Chen J, Lozach J, Garcia EW, Barnes B, Luo S, Mikoulitch I, Zhou L, Schroth G, Fan JB (2008) Highly sensitive and specific microRNA expression profiling using BeadArray technology. Nucleic Acids Res 36:e87

4. Cheng A, Li M, Liang Y, Wang Y, Wong L, Chen C, Vlassov AV, Magdaleno S (2009) Stem-loop RT-PCR quantification of siRNAs in vitro and in vivo. Oligonucleotides 19:203–208

5. Crawford GE, Davis S, Scacheri PC, Renaud G, Halawi MJ, Erdos MR, Green R, Meltzer PS, Wolfsberg TG, Collins FS (2006) DNase-chip: a high-resolution method to identify DNase I hypersensitive sites using tiled microarrays. Nat Methods 3:503–509

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