Characterization of an efficient CRISPR-iCas9 system in Yarrowia lipolytica for the biosynthesis of carotenoids
Author:
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s00253-023-12731-w.pdf
Reference45 articles.
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2. Baisya D, Ramesh A, Schwartz C, Lonardi S, Wheeldon I (2022) Genome-wide functional screens enable the prediction of high activity CRISPR-Cas9 and -Cas12a guides in Yarrowia lipolytica. Nat Commun 13(1):922. https://doi.org/10.1038/s41467-022-28540-0
3. Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H (2015) Homology-integrated CRISPR–Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae. ACS Synth Biol 4(5):585–594. https://doi.org/10.1021/sb500255k
4. Blazeck J, Liu L, Knight R, Alper HS (2013) Heterologous production of pentane in the oleaginous yeast Yarrowia lipolytica. J Biotechnol 165(3-4):184–194. https://doi.org/10.1016/j.jbiotec.2013.04.003
5. Cao X, Lv Y-B, Chen J, Imanaka T, Wei L-J, Hua Q (2016) Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction. Biotechnol Biofuels 9. https://doi.org/10.1186/s13068-016-0626-7
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