A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method-Reference-Cited by-同舟云学术

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A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method

Published:2020-03-09 Issue:9 Volume:104 Page:3993-4003
ISSN:0175-7598
Container-title:Applied Microbiology and Biotechnology
language:en
Short-container-title:Appl Microbiol Biotechnol

Author:

Dong Mengjie,Wang Fei,Li Qingqing,Han Rui,Li Aitao,Zhai Chao,Ma Lixin

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

http://link.springer.com/content/pdf/10.1007/s00253-020-10477-3.pdf

Reference32 articles.

1. AlMalki FA, Flemming CS, Zhang J, Feng M, Sedelnikova SE, Ceska T, Rafferty JB, Sayers JR, Artymiuk PJ (2016) Direct observation of DNA threading in flap endonuclease complexes. Nat Struct Mol Biol 23:640–646. https://doi.org/10.1038/nsmb.3241

2. Ceska TA, Sayers JR, Stier G, Suck D (1996) A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease. Nature 382:90–93. https://doi.org/10.1038/382090a0

3. Chow RD, Wang G, Ye L, Codina A, Kim HR, Shen L, Dong MB, Errami Y, Chen S (2019) In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens. Nat Methods 16:405–408. https://doi.org/10.1038/s41592-019-0371-5

4. Ellis HM, Yu D, DiTizio T, Court DL (2001) High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides. Proc Natl Acad Sci U S A 98:6742–6746. https://doi.org/10.1073/pnas.121164898

5. Endo A, Masafumi M, Kaya H, Toki S (2016) Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida. Sci Rep 6:38169. https://doi.org/10.1038/srep38169

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