CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes
Author:
Funder
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s10529-023-03363-1.pdf
Reference26 articles.
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2. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640–6645
3. Dong M, Wang F, Li Q, Han R, Li A, Zhai C, Ma L (2020) A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method. Appl Microbiol Biotechnol 104:3993–4003
4. Ellis HM, Yu D, DiTizio T, Court DL (2001) High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides. Proc Natl Acad Sci U S A 98:6742–6746
5. Gao X, Zhang F, Wu M, Wu Z, Shang G (2019) Production of N-acetyl-D-neuraminic acid by whole cells expressing Bacteroides thetaiotaomicron N-acetyl-D-glucosamine 2-epimerase and Escherichia coli N-acetyl-D-neuraminic acid aldolase. J Agric Food Chem 67:6285–6291
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