Abstract
AbstractPandoraea sp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damage of the lungs. The molecular mechanisms underlying the high pathogenicity of Pandoraea species are still largely unknown. As Gram-negatives, Pandoraea sp. express lipopolysaccharides (LPS) whose recognition by the host immune system triggers an inflammatory response aimed at the bacterial eradication from the infected tissues. The degree of the inflammatory response strongly relies on the fine structure of the LPS and, in particular, of its glycolipid moiety, i.e. the lipid A. Here we report the structure of the lipid A isolated from the LPS of a chronic strain of P. pulmonicola (RL 8228), one of the most virulent identified so far among the Pandoraea species. Our data demonstrated that the examined chronic strain produces a smooth-type LPS with a complex mixture of hypoacylated lipid A species displaying, among other uncommon characteristics, the 2-hydroxylation of some of the acyl chains and the substitution by an additional glucosamine on one or both the phosphate groups.
Funder
H2020 Marie Skłodowska-Curie Actions
Compagnia di San Paolo
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Biochemistry
Reference33 articles.
1. Rafeeq, M.M., Murad, H.A.S.: Cystic fibrosis: current therapeutic targets and future approaches. J. Transl. Med. 15, 84 (2017)
2. Sockrider, M.M., Ferkol, T.W.: Twenty Facts About Cystic Fibrosis. Am. J. Respir. Crit. Care Med. 196(12), P23–P24 (2017)
3. Mahenthiralingam, E.: Emerging cystic fibrosis pathogens and the microbiome. Paediatr. Respir. Rev. 15, 13–15 (2014)
4. Caraher, E., Collins, J., Herbert, G., Murphy, P.G., Gallagher, C.G., Crowe, M.J., Callaghan, M., McClean, S.: Evaluation of in vitro virulence characteristics of the genus Pandoraea in lung epithelial cells. J. Med. Microbiol. 57, 15–20 (2008)
5. Costello, A., Herbert, G., Fabunmi, L., Schaffer, K., Kavanagh, K.A., Caraher, E.M., Callaghan, M., McClean, S.: Virulence of an Emerging Respiratory Pathogen, Genus Pandoraea, in vivo and Its Interactions with Lung Epithelial Cells. J. Med. Microbiol. 60(Pt 3), 289–299 (2011)
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献