Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli-Reference-Cited by-同舟云学术

Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli

Published:2016-09-27 Issue: Volume: Page:49-61
ISSN:1064-3745
Container-title:Methods in Molecular Biology
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Author:

Zhong Chao,You Chun,Wei Ping,Zhang Yi-Heng Percival

Publisher

Springer New York

Link

http://link.springer.com/content/pdf/10.1007/978-1-4939-6343-0_4

Reference12 articles.

1. Moxon ER, Higgins CF (1997) E. coli gene sequence - a blueprint for life. Nature 389:120–121

2. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York

3. Lund AH, Duch M, Pedersen FS (1996) Increased cloning efficiency by temperature-cycle ligation. Nucleic Acids Res 24:800–801

4. Young TS, Schultz PG (2010) Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem 285:11039–11044

5. Li CA, Evans RM (1997) Ligation independent cloning irrespective of restriction site compatibility. Nucleic Acids Res 25:4165–4166

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