Abstract
AbstractCanA from Pyrodictium abyssi forms a heat-resistant organic hollow-fiber network together with CanB and CanC. An N-terminally truncated construct of CanA (K1-CanA) gave NMR spectra of good quality that could be assigned by three-dimensional NMR methods on 15N and 13C–15N enriched protein. We assigned the chemical shifts of 96% of all backbone 1HN atoms, 98% of all backbone 15N atoms, 100% of all 13Cα atoms, 100% of all 1Hα atoms, 90% of all 13C′ atoms, and 100% of the 13Cβ atoms. Two short helices and 10 β-strands are estimated from an analysis of the chemical shifts leading to a secondary structure content of K1-CanA of 6% helices, 44% β-pleated sheets, and 50% coils.
Funder
Deutsche Forschungsgemeinschaft
FCI
Publisher
Springer Science and Business Media LLC
Subject
Biochemistry,Structural Biology
Reference19 articles.
1. Budisa N, Steipe B, Demange P, Eckerskorn C, Kellermann J, Huber R (1995) High-level biosynthetic substitution of methionine in proteins by its analogs 2-aminohexanoic acid, selenomethionine, telluromethionine and ethionine in Escherichia coli. Eur J Biochem 230:788–796
2. Deininger W (1994) Untersuchungen zur hitzeabhängigen expression des Thermosoms bei Pyrodictium sowie Isolierung und Charakterisierung eines neuen Vertreters der Art Pyrodictium (TAG11) aus der Tiefsee. Diploma thesis, University of Regensburg.
3. Gronwald W, Kalbitzer HR (2004) Automated structure determination of proteins by NMR spectroscopy. Progr NMR Spectr 44:33–96
4. Harsch T, Schneider P, Kieninger B, Donaubauer H, Kalbitzer HR (2017) Stereospecific assignment of the asparagine and glutamine side chain amide protons in proteins from chemical shift analysis. J Biomol NMR 67:157–164
5. Horn C, Paulmann B, Kerlen G, Junker N, Huber H (1999) In vivo observation of cell division of anaerobic hyperthermophiles by using a high-intensity dark-field microscope. J Bacteriol 181:5114–5118
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