In Vivo Observation of Cell Division of Anaerobic Hyperthermophiles by Using a High-Intensity Dark-Field Microscope-Reference-Cited by-同舟云学术

In Vivo Observation of Cell Division of Anaerobic Hyperthermophiles by Using a High-Intensity Dark-Field Microscope

Published:1999-08-15 Issue:16 Volume:181 Page:5114-5118
ISSN:1098-5530
Container-title:Journal of Bacteriology
language:en
Short-container-title:J. Bacteriol.

Author:

Horn Christian¹,Paulmann Bernd¹,Kerlen Gertraude²,Junker Norbert³,Huber Harald¹

Affiliation:

1. Archaeenzentrum, Universität Regensburg, 93053 Regensburg,1

2. Institut für den wissenschaftlichen Film, 37075 Göttingen,2 and

3. Olympus Optical Co. (Europe) GmbH, 20097 Hamburg,3 Germany

Abstract

ABSTRACT To study growth and cell division of anaerobic hyperthermophilic archaea in vivo, a cultivation technique using glass capillaries was developed. At temperatures of 90 to 98°C, at least 10 successive cell divisions of Pyrodictium abyssi TAG 11 were documented. Cells divide by binary fission. Visualized under a modified dark-field microscope, the formation of cannulae, which finally connected all cells, was observed. The cannulae elongated at 1.0 to 1.5 μm/min and reached final lengths of between 30 and 150 μm. A “snapping division”-like mode of cell fission was discovered forThermoproteus tenax.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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