Metabolic and kinetic studies of hybridomas in exponentially fed-batch cultures using T-flasks

Author:

Higareda Ana E.,Possani Lourival D.,Ram�rez Octavio T.

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Clinical Biochemistry,Biomedical Engineering,Bioengineering,Biotechnology

Reference35 articles.

1. Boraston R, Thompson PW, Garland S and Birch JR (1984) Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture. Dev. Biol. Stand. 55: 103?104

2. Chevalot I and Marc A (1993) Interest of fed-batch culture for the production of a membrane-bound protein by an adherent animal cell. Biotech. Letts. 15: 791?796.

3. Esener AA, Roels JA and Kossen NWF (1981) Fed-batch culture: modeling and applications in the study of microbial energetics. Biotech. Bioeng. 23: 1851?1871.

4. Flickinger MC, Goebel NK and Bohn MA (1990) Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth. Bioproc. Eng. 5: 155?164.

5. Flickinger MC, Bibila TA and Kitchin K (1992) Prediction and experimental investigation of the effect of altered peptide chain assembly rate on antibody secretion using a structured model of antibody synthesis and secretion. In: Ladisch MR and Bose A (ed.) Harnessing Biotechnology for the 21st Century. (pp. 345?350) American Chemical Society.

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