Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater
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Published:2023-09-21
Issue:48
Volume:30
Page:106660-106670
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ISSN:1614-7499
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Container-title:Environmental Science and Pollution Research
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language:en
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Short-container-title:Environ Sci Pollut Res
Author:
Cutrupi FrancescaORCID, Rossi Michele, Cadonna Maria, Poznanski Elisa, Manara Serena, Postinghel Mattia, Palumbi Giulia, Bellisomi Marta, Nicosia Elena, Allaria Giorgia, Dondero Lorenzo, Veneri Carolina, Mancini Pamela, Ferraro Giusy Bonanno, Rosa Giuseppina, Suffredini Elisabetta, Foladori Paola, Grasselli Elena
Abstract
AbstractCrucial information on the pandemic’s spread has been gathered by monitoring the trend of SARS-CoV-2 in wastewater. This surveillance has highlighted that the initial concentration is a critical step of the analytical procedure due to the low viral titer that may be present in this matrix. This paper presents the results of the evaluation of two different wastewater concentration protocols to determine the most efficient and cost-effective. The two methods tested were the following: (a) a biphasic separation system with PEG-dextran and (b) a PEG/NaCl precipitation protocol. Other aspects of the detection method were also investigated including the influence of storage temperature on virus recovery and the heat treatment of pasteurization, which aims to make samples safer for operators and the environment. The PEG/NaCl precipitation method was found to perform better than the biphasic separation system, allowing for more sensitive identification of the presence of the virus and the detection of a higher viral titer than that identified with the biphasic separation in all results. Storage of the samples at 4.3±0.2°C for up to 3 weeks did not adversely affect the virus titer and the pasteurization pre-treatment increases operator safety and maintains the identification of the viral concentration.
Graphical abstract
Funder
Università degli Studi di Trento
Publisher
Springer Science and Business Media LLC
Subject
Health, Toxicology and Mutagenesis,Pollution,Environmental Chemistry,General Medicine
Reference35 articles.
1. Ahmed W, Angel N, Edson J, Bibby K, Bivins A, O’Brien JW, Choi PM, Kitajima M, Simpson SL, Li J, Tscharke B, Verhagen R, WJM S, Zaugg J, Dierens L, Hugenholtz P, Thomas KV, Mueller JF (2020) First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: a proof of concept for the wastewater surveillance of COVID-19 in the community. Sci Total Environ 728:138764. https://doi.org/10.1016/j.scitotenv.2020.138764 2. Cutrupi F, Cadonna M, Manara S, Postinghel M, La Rosa G, Suffredini E, Foladori P (2022) The wave of the SARS-CoV-2 Omicron variant resulted in a rapid spike and decline as highlighted by municipal wastewater surveillance. Environ Technol Innov 28:102667. https://doi.org/10.1016/j.eti.2022.102667 3. Dimitrakopoulos L, Kontou A, Strati A, Galani A, Kostakis M, Kapes V, Lianidou E, Thomaidis N, Markou A (2022) Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR. Case Stud Chem Environ Eng 6:100224. https://doi.org/10.1016/j.cscee.2022.100224 4. ggplot2 (2016) https://ggplot2.tidyverse.org/. Accessed 2.13.23x 5. He X, EHY L, Wu P, Deng X, Wang J, Hao X, Lau YC, Wong JY, Guan Y, Tan X, Mo X, Chen Y, Liao B, Chen W, Hu F, Zhang Q, Zhong M, Wu Y, Zhao L et al (2020) Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med 26:672–675. https://doi.org/10.1038/s41591-020-0869-5
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