Affiliation:
1. Zymergen Inc. 5980 Horton Street, Suite #105 94608 Emeryville CA USA
Abstract
Abstract
While design and high-throughput build approaches in biotechnology have increasingly gained attention over the past decade, approaches to test strain performance in high-throughput have received less discussion in the literature. Here, we describe how fermentation characterization can be used to improve the overall efficiency of high-throughput DBTAL (design-build-test-analyze-learn) cycles in an industrial context. Fermentation characterization comprises an in-depth study of strain performance in a bioreactor setting and involves semi-frequent sampling and analytical measurement of substrates, cell densities and viabilities, and (by)products. We describe how fermentation characterization can be used to (1) improve (high-throughput) strain design approaches; (2) enable the development of bench-scale fermentation processes compatible with a wide diversity of strains; and (3) inform the development of high-throughput plate-based strain testing procedures for improved performance at larger scales.
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
13 articles.
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