Affiliation:
1. grid.8385.6 0000 0001 2297 375X Institute of Bio- and Geosciences, IBG-1: Biotechnology Forschungszentrum Jülich GmbH Jülich Germany
2. grid.1957.a 0000 0001 0728 696X Institute of Biotechnology RWTH Aachen University Aachen Germany
3. grid.1957.a 0000 0001 0728 696X Computational Systems Biotechnology RWTH Aachen University Aachen Germany
Abstract
Abstract
Limited throughput represents a substantial drawback during bioprocess development. In recent years, several commercial microbioreactor systems have emerged featuring parallelized experimentation with optical monitoring. However, many devices remain limited to batch mode and do not represent the fed-batch strategy typically applied on an industrial scale. A workflow for 32-fold parallelized microscale cultivation of protein secreting Corynebacterium glutamicum in microtiter plates incorporating online monitoring, pH control and feeding was developed and validated. Critical interference of the essential media component protocatechuic acid with pH measurement was revealed, but was effectively resolved by 80% concentration reduction without affecting biological performance. Microfluidic pH control and feeding (pulsed, constant and exponential) were successfully implemented: Whereas pH control improved performance only slightly, feeding revealed a much higher optimization potential. Exponential feeding with µ = 0.1 h−1 resulted in the highest product titers. In contrast, other performance indicators such as biomass-specific or volumetric productivity resulted in different optimal feeding regimes.
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
10 articles.
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