Affiliation:
1. grid.411410.1 000000008822034X Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering Hubei University of Technology 430068 Wuhan People’s Republic of China
2. grid.261128.e 0000000090038934 Department of Biological Sciences Northern Illinois University 60115 DeKalb IL USA
Abstract
Abstract
d(−)-lactic acid is needed for manufacturing of stereo-complex poly-lactic acid polymer. Large scale d-lactic acid fermentation, however, has yet to be demonstrated. A genetically engineered Escherichia coli strain, HBUT-D, was adaptively evolved in a 15% calcium lactate medium for improved lactate tolerance. The resulting strain, HBUT-D15, was tested at a lab scale (7 L) by fed-batch fermentation with up to 200 g L−1 of glucose, producing 184–191 g L−1 of d-lactic acid, with a volumetric productivity of 4.38 g L−1 h−1, a yield of 92%, and an optical purity of 99.9%. The HBUT-D15 was then evaluated at a semi-industrial scale (30 m3) via fed-batch fermentation with up to 160 g L−1 of glucose, producing 146–150 g L−1 of d-lactic acid, with a volumetric productivity of 3.95–4.29 g L−1 h−1, a yield of 91–94%, and an optical purity of 99.8%. These results are comparable to that of current industrial scale l(+)-lactic acid fermentation.
Funder
Natural Science Foundation of Hubei Province
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
18 articles.
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