Abstract
AbstractLipoxygenase (LOX) activity is closely related to wheat processing and storage quality. In the present research, ten wheat cultivars were used to compare the effects of genotype, location, year, and their interactions on the LOX activity. Furthermore, 123 wheat cultivars were evaluated for LOX activity with 192 simple sequence repeat (SSR) markers and to identify elite alleles related to LOX activity. The results indicated that LOX activity was highly affected by genotype (variety) than that by the location. A total of 22 SSR molecular marker loci with a significant or very significant correlation with LOX activity were identified on performing association analysis. In 3 years, only one molecular marker locus associated with LOX activity was detected (WMC488); in 2 years, seven molecular marker loci were detected, while in only 1 year, the other 14 molecular marker loci were detected. A total of 7 and 6 marker loci significantly related to LOX activity accounting for 31.2% and 27.2%, respectively, were located in homologous groups 4 and 5, and group 7. This research provided the theoretical basis and the markers for molecular-assisted wheat breeding that facilitate the breeding process in the processing and storage quality of grains.
Funder
University Natural Science Research Project of Anhui Province
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Agronomy and Crop Science,Physiology
Reference25 articles.
1. Ahmed I, Islam M, Arshad W, Mannan A, Ahmad W, Mirza B (2009) High-quality plant DNA extraction for PCR: an easy approach. J Appl Genet 50(2):105–107
2. Barone R, Briante R, D’Auria S, Febbraio F, Vaccaro C, Del Giudice L, Borrelli GM, Di Fonzo N, Nucci R (1999) Purification and characterization of a lipoxygenase enzyme from durum wheat semolina. J Agric Food Chem 47(5):11924–11931
3. Borrelli GM, De Leonardis AM, Fares C, Platani C, Di Fonzo N (2003) Effects of modified processing conditions on oxidative properties of semolina dough and pasta. Cereal Chem 80(2):225–231
4. Borrelli GM, De Leonardis AM, Platani C, Troccoli A (2008) (2007). Contribution along durum wheat kernel of the components involved in semolina color. J Cereal Sci 76:1–9
5. Boyington JC, Gaffney BJ, Arnzel LM (1993) The three-dimensional structure of an arachidonic acid 15-lipoxygenase. Science 260:1482–1486
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献