Author:
Cheng B.,Ding Y. Q.,Gao X.,Cao N.,Xin Z. H.,Zhang L. Y.
Abstract
AbstractKnowledge of genetic diversity and genes conferring disease resistance is essential for the effective use of wheat germplasm resistant to powdery mildew in breeding programs in southwest China. In this study, a collection of 140 common wheat varieties was evaluated for their resistance to powdery mildew in four different environments. Thirty-six varieties (25.71%) showed resistance in all four environments, and most of these varieties originated from the Guizhou Province. This collection was also scanned for molecular markers closely linked to the powdery mildew resistance genes. Most of the wheat varieties (79.86%) carried the resistance Pm30 gene locus. Genotyping by sequencing (GBS) was used to screen our collection against the whole genome. Finally, 6864 informative SNP markers were used to construct a consensus tree with 1000 bootstraps, which showed two groups based on their origins and the presence of the Pm21 gene locus. Our results provide useful information for breeding wheat resistant to powdery mildew in China, and they will likely contribute to the identification of new resistance genes in the future.
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Agronomy and Crop Science,Physiology
Reference32 articles.
1. Belova T, Zhan B, Wright J, Caccamo M, Asp T, Simková H, Kent M, Bendixen C, Panitz F, Lien S, Doležel J, Olsen OA, Sandve SR (2013) Integration of mate pair sequences to improve shotgun assemblies of flow-sorted chromosome arms of hexaploid wheat. BMC Genom 14:222
2. Bennett FGA (1984) Resistance to powdery mildew in wheat: a review of its use in agriculture and breeding programs. Plant Pathol 33:279–300
3. Bie T, Zhao R, Zhu S, Chen S, Cen B, Zhang B, Gao D, Jiang Z, Chen T, Wang L, Wu R, He H (2015) Development and characterization of marker MBH1 simultaneously tagging genes Pm21 and PmV conferring resistance to powdery mildew in wheat. Mol Breed 35:189
4. Cao X, Zhou Y, Duan X, Song Y, He W, Ding K, Wang B, Xia X (2010) Postulation of wheat powdery mildew resistance genes in 101 wheat cultivars (lines) from major wheat-cultivating regions in china. J Triticeae Crops 30:948–953 (in Chinese with English abstract)
5. Cao A, Xing L, Wang X, Yang X, Wang W, Sun Y, Qian C, Ni J, Chen Y, Liu D, Wang X, Chen P (2011) Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. P Natl Acad Sci USA 108:7727–7732
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