The effects of using hydrolyzed feather meal, amino acids, and probiotics in the diet of juvenile rainbow trout on growth, digestibility, and expression of growth-related genes

Author:

Metin Ömer,Yildiz Mustafa,Eldem Vahap,Adabi Shahram Golzar

Abstract

AbstractThe study investigated the effect of replacing fishmeal by varying levels of hydrolyzed feather meal (HFM) as well as dietary probiotic supplementation on growth performance, digestibility, histology, and gene expression of juvenile rainbow trout (Oncorhynchus mykiss). Five iso-nitrogenous (average of 50.60% crude protein), iso-energetic (average of 21.79 kJ/g), and iso-lipidic (average of 21.86% crude lipid) diets were formulated and fed to triplicate groups of juvenile rainbow trout weighing 29.65 ± 0.39 g (mean ± SD) for 75 days. Dietary treatments included one control (50% fishmeal) and four experimental diets, each containing 2 g/kg probiotics (Lactobacillus plantarum and Bacillus subtilis). The experimental diets included HFM30 (150 g/kg HFM, 9 g/kg lysine, and 4 g/kg methionine), HFM35 (175 g/kg HFM, 11 g/kg lysine, and 5 g/kg methionine), HFM40 (200 g/kg HFM, 13 g/kg lysine, and 6 g/kg methionine), and HFM45 (225 g/kg HFM, 15 g/kg lysine, and 7 g/kg methionine). Up to 40% replacement of fishmeal with HFM showed no significant difference compared to control group in growth performance (P > 0.05). Feed utilization of HFM45 was slightly lower than control and all the other experimental groups. There was no statistically significant difference in FCR value between HFM40 and control groups (P > 0.05). The apparent digestibility coefficients (ADCs) for protein and amino acids were very high in all groups (P < 0.05). Intestinal mucosa and sub‐mucosa layers of experimental fish showed general tissue integrity with no signs of tissue necrosis. For intestinal morphology, where 45% HFM replacement was applied, villus length to crypt depth (VL to CD) and villus surface area (SA) decreased by 46.01% and 44.44%, respectively, compared to the control group (P < 0.05). This situation shows that intestinal morphology can be damaged when the highest level (HFM45) of feather meal is used. Dietary HFM upregulated growth-related genes such as IGF-II, IGFBP-1b, IGFr1a, GDF9, and FGF2 and downregulated GHR-I and IGF-I. Overall, inclusion of HFM at 40% showed similar results to control group in juvenile rainbow trout feeding.

Funder

Istanbul University

Publisher

Springer Science and Business Media LLC

Reference79 articles.

1. Abdel-Latif HM, Chaklader R, Shukry M, Ahmed HA, Khallaf MA (2023) A multispecies probiotic modulates growth, digestive enzymes, immunity, hepatic antioxidant activity, and disease resistance of Pangasianodon hypophthalmus fingerlings. Aquaculture 563:738948. https://doi.org/10.1016/j.aquaculture.2022.738948

2. Abo-Norag MA, El-Shenawy AM, Fadl SE, Abdo W, Gad DM, Rashed MA, Prince A (2018) Effect of phytase enzyme on growth performance, serum biochemical alteration, immune response and gene expression in Nile tilapia. Fish Shellfish Immunol 80:97–108. https://doi.org/10.1016/j.fsi.2018.05.051

3. Adelina A, Feliatra F, Siregar YI, Putra I, Suharman I (2021) Use of chicken feather meal fermented with Bacillus subtilis in diets to increase the digestive enzymes activity and nutrient digestibility of silver pompano Trachinotus blochii (Lacepede, 1801). F1000Research 10:25. https://doi.org/10.12688/f1000research.26834.2

4. Aksnes A, Hope B, Albrektsen S (2006) Size-fractionated fish hydrolysate as feed ingredient for rainbow trout (Oncorhynchus mykiss) fed high plant protein diets. II: flesh quality, absorption, retention and fillet levels of taurine and anserine. Aquaculture 261(1):318–326. https://doi.org/10.1016/j.aquaculture.2006.07.026

5. Alam MS, Watanabe WO, Carroll PM, Gabel JE, Corum M, Seaton PJ, Wedegaertner T, Rathore KS, Dowd MK (2018) Evaluation of genetically-improved (glandless) and genetically-modified low-gossypol cottonseed meal as alternative protein sources in the diet of juvenile southern flounder Paralichthys lethostigma reared in a recirculating aquaculture system. Aquaculture 489:36–45. https://doi.org/10.1016/j.aquaculture.2018.02.006

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3