Abstract
AbstractThe gases used: ethylene (C2H4), hydrogen cyanide (HCN) and nitric oxide (NO) showed a high activity as inductors of germination in primary dormant or non-dormant seeds exposed to stress or suboptimal temperatures. So far, research on the role of ethylene, hydrogen cyanide and nitric oxide has involved these gases during seed germination. This work describes gas-priming as a novel method for treating air dry seeds of the genus Amaranthus serving as a model. Effects of C2H4, HCN or NO applied to dry seeds were similar to those obtained when the gases were used during seed germination for an identical period of time. Application of the gases to air dry seeds presents a new opportunity to study the role of those gases in germination of dormant and non-dormant seeds and to constrain effects of the gases from time 0. The prolonged treatment time for dry seeds (24 h) is convenient because if the treatment is started in the morning, there is sufficient time the next day for further experiments to begin, e.g. germination test in water or in solutions of other compounds. Moreover, it is important that the gas-treated seeds can be stored or transported prior to use. The gas-priming method allows to prepare treated seed samples which can be used in experiments either immediately or after storage in open air or in a closed container. Gas-priming with C2H4, HCN and NO is a simple and useful treatment of air-dried seeds, which opens up new useful possibilities for basic research on the role of those gases in releasing dormancy and seed germination of various plant species. In addition, the method may prove very useful in horticulture and agriculture in improving germination of gas-sensitive seeds.
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Agronomy and Crop Science,Physiology
Reference34 articles.
1. Arc E, Sechet J, Corbineau F, Rajjou L, Marion-Poll A (2013) ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Front Plant Sci 63:1–19
2. Bethke PC, Libourel IGL, Aoyama N, Chung YY, Still DW, Jones RL (2007a) The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy. Plant Physiol 143:1173–1188
3. Bethke PC, Libourel IGL, Jones RL (2007b) Nitric oxide in seed dormancy and germination. Annu Plant Rev 27:153–175
4. Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066
5. Bewley JD, Bradford KJ, Hilhorst HWM, Nonogaki H (2013) Seeds: physiology of development, germination and dormancy. Springer, New York
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献