A high-affinity folate binding protein in human urine. Radioligand binding characteristics, immunological properties and molecular size-Reference-Cited by-同舟云学术

A high-affinity folate binding protein in human urine. Radioligand binding characteristics, immunological properties and molecular size

Published:1989-02-01 Issue:1 Volume:9 Page:93-97
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Hansen Steen Ingemann¹,Holm Jan²,Høier-Madsen Mimi³

Affiliation:

1. Department of Clinical Chemistry, Central Hospital Hillerød, Hillerød, 3400 Denmark

2. Department of Clinical Chemistry, Central Hospital Nykøbing Falster, Nykøbing Falster, 4800 Denmark

3. Laboratory for Autoimmune Serology, State Serum Institute, 2300 S Copenhagen, Denmark

Abstract

High-affinity binding of [3H]folate in human urine displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. By means of a two-site enzyme-linked immunosorbent assay (ELISA) with rabbit antibodies against the low molecular weight folate binding protein from human milk, we measured folate binding protein concentrations in the range of 0.51 to 4.13 nM in urine samples from 16 apparently healthy individuals. Ultrogel AcA 44 chromatography of the urine showed that immunoreactive and radioligand bound folate binding protein coeluted in one large peak (Mr∼25,000).

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/9/1/93/472899/bsr0090093.pdf

Reference11 articles.

1. Hansen, S. I., Holm, J. and Lyngbye, J. (1980)IRCS Med. Sci. 8:846?847.

2. Høier-Madsen, M., Hansen, S. I. and Holm, J. (1987)Biosci. Rep. 7:553?557.

3. Holm, J., Hansen, S. I. and Lyngbye, J. (1980)Biochim. Biophys. Acta 629:539?545.

4. Svendsen, I., Martin, B., Pedersen, T. G., Hansen, S. I., Holm, J. and Lyngbye, J. (1979)Carlsberg Res. Commun. 44:89?99.

5. Svendsen, I., Hansen, S. I., Holm, J. and Lyngbye, J. (1982)Carlsberg Res. Commun. 47:371?376.

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