Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA)-Reference-Cited by-同舟云学术

Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA)

Published:1987-07-01 Issue:7 Volume:7 Page:553-557
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Høier-Madsen Mimi¹,Hansen Steen Ingemann²,Holm Jan³

Affiliation:

1. Laboratory for Autoimmune Serology, State Serum Institute, Copenhagen, 2300 S Denmark

2. Department of Clinical Chemistry, Central Hospital Hillerød, Hillerød, 3400 Denmark

3. Department of Clinical Chemistry, Central Hospital Nykøbing Falster, Nykøbing Falster, 4800 Denmark

Abstract

Antibodies to a low molecular weight folate binding protein isolated from human milk were raised in rabbits and used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for immunological characterization of human folate binding proteins (FBPs). The high and low molecular weight FBPs from human milk were immunologically indistinguishable. Furthermore, the FBPs in human urine and cerebrospinal fluid showed a cross-reactivity of 70% and 30%, respectively. No cross-reactivity of the FBP from cow's milk was observed.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/7/7/553/472064/bsr0070553.pdf

Reference9 articles.

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3. Geisler, C. and Høier-Madsen, M. (1985).J. Immunol. Methods 80:211?219.

4. Høier-Madsen, M., Hansen, S. I. and Holm, J. (1986).Biosci. Rep. 6:895?900.

5. Bayer, E. A. and Wilchek, M. (1980).Methods Biochem. Anal. 26:1?45.

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