Process development for efficient pectin extraction from tobacco residues and its characterisation

Author:

Bedzo Oscar K. K.,Gottumukkala Lalitha D.,Sasso Giuseppe Lo,Kaminski Kacper,Schlage Walter,Goffman Fernando,Ivanov Nikolai,Hoeng Julia,Hayes Daniel J.

Abstract

AbstractThis study presents a method for effective pectin extraction from the laminae of three tobacco varieties as a means of biomass valorisation. Two pre-treatment methods (cold ethanol vs. accelerated solvent extraction [ASE] with ethanol) were compared for their capacities to produce a high pectin yield. Enzymatic extraction of pectin was also tested as a green extraction procedure and compared to the acid extraction approach. The optimisation experiments revealed that cold ethanol extraction followed by acid hydrolysis is the most convenient method for pectin extraction; the optimal set of conditions for hydrolysis were identified as 90°C, pH 1.5, and 4 h of extraction. Applying these optimised conditions to the three Nicotiana rustica tobacco varieties yielded pectin recoveries of 66.2%, 57.8%, and 56.7% from the NRT63, Bakoum Miena, and NRT61 samples, respectively. Tobacco pectins were found to have a medium molecular weight and low methoxy content. These results highlight the potential of tobacco residues as feedstock for to produce pectin with dietary applications.

Publisher

Springer Science and Business Media LLC

Subject

Renewable Energy, Sustainability and the Environment

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