Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes—Freshly isolated cells, cell suspensions, and primary monolayer cultures

Author:

Jauregui Hugo O.,Hayner Nancy T.,Driscoll James L.,Williams-Holland Rhonda,Lipsky Milton H.,Galletti Pierre M.

Publisher

Springer Science and Business Media LLC

Subject

Plant Science,Biotechnology,Plant Science,Biotechnology

Reference41 articles.

1. Seglen, P. O. Preparation of isolated rat liver cells. Prescott, D. M. ed. Methods in cell biology. Vol. 13. New York: Academic Press; 1976: 29–83.

2. Gebhart, R.; Bellemann, P.; Mecke, D. Metabolic and enzymatic characteristics of adult rat liver parenchymal cells in non-proliferating primary monolayer cultures. Exp. Cell Res. 112: 431–441; 1978.

3. Fry, J. C.; Jones, C. A.; Weibkin, P.; Bellemann, P.; Bridges, J. W. The enzymic isolation of adult rat hepatocytes in a functional and viable state. Anal. Biochem. 71: 341–350; 1976.

4. Bissell, D.; Guzelian, P. S. Phenotypic stability of adult rat hepatocytes in primary monolayer culture. Borek, C.; Williams, G. M. eds., Differentiation and carcinogenesis in liver cell cultures. Vol. 349, New York: New York Academy of Sciences; 1980: 85–98.

5. Krebs, H. A.; Lund, P.; Edwards, M. Criteria of metabolic competence of isolated hepatocytes. Reid, E. ed. Cell populations. England: Ellis Horwood Limited; 1979: 1–6.

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